Monoclonal Anti-RAD18 antibody produced in mouse
SIGMA/WH0056852M1 - clone 3H7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-RAD18 homolog (S. cerevisiae); Anti-RNF73
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell. [antibody concentration 25 μg/mL]
Western Blotting RAD18 monoclonal antibody, clone 3H7 Western Blot analysis of RAD18 expression in HeLa S3 NE.
Enhanced Validation-RNAi Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi. Blot probed with RAD18 monoclonal antibody, clone 3H7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody, clone 3H7. Lanes Lane 1: RAD18 transfected lysate (56.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting RAD18 monoclonal antibody, clone 3H7. Western Blot analysis of RAD18 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged RAD18 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_020165   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NS91 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. (provided by RefSeq) |
| Immunogen: | RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EKEIDEIHSKYRKKHKSEFQLLVDQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |