Monoclonal Anti-C1GALT1 antibody produced in mouse
SIGMA/WH0056913M1 - clone 1F1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-C1GALT; Anti-Tsynthase; Anti-core 1 synthase, glycoprotein-
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to C1GALT1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. [antibody concentration 3 μg/mL]
Western Blotting C1GALT1 monoclonal antibody, clone 1F1 Western Blot analysis of C1GALT1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged C1GALT1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_020156   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NS00 |
| Biochem/physiol Actions: | Glycoprotein-N- acetylgalactosamine 3-β- galactosyltransferase 1 (C1GALT1) functions in the addition of galactose onto GalNAc residues of IgA1. Thus, it generates the core 1 O-glycan Galβ1-3GalNAα1-SerThr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. A deficiency of C1GALT1 results in IgA nephropathy, Tn syndrome and also Henoch-Schönlein purpura. It increases the proliferation of hepatocellular carcinoma cells by regulating MET glycosylation and dimerization. C1GALT1 also regulates angiogenesis, kidney development and thrombopoiesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glycoprotein-N- acetylgalactosamine 3-β- galactosyltransferase 1 (C1GALT1) is localized in the Golgi apparatus. The gene encoding it contains three exons spanning 10 kb. It is located on chromosome 7p14-p13. |
| Immunogen: | C1GALT1 (NP_064541, 264 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ETFHPFVPEHHLIKGYLPRTFWYWN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |