Monoclonal Anti-AGTRAP antibody produced in mouse
SIGMA/WH0057085M2 - clone 1G2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ATRAP; Anti-angiotensin II receptor-associated protein
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to AGTRAP on formalin-fixed paraffin-embedded human prostate. [antibody concentration 0.8 μg/mL]
Western Blotting Western Blot analysis of AGTRAP expression in transfected 293T cell line by AGTRAP monoclonal antibody, clone 1G2. Lanes Lane 1: AGTRAP transfected lysate (16.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting AGTRAP monoclonal antibody, clone 1G2 Western Blot analysis of AGTRAP expression in HeLa S3 NE.
Enhanced Validation-RNAi Western blot analysis of AGTRAP over-expressed 293 cell line, cotransfected with AGTRAP Validated Chimera RNAi. Blot probed with AGTRAP monoclonal antibody, clone 1G2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (31.46 kDa).
ELISA Detection limit for recombinant GST tagged AGTRAP is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_020350   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q6RW13 |
| General description: | This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | AGTRAP (NP_065083, 108 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence HMYRERGGELLVHTGFLGSSQDRSA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |