Monoclonal Anti-SMURF1 antibody produced in mouse
SIGMA/WH0057154M1 - clone 1D7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-KIAA1625; Anti-SMAD specific E3 ubiquitin protein ligase 1
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SMURF1 monoclonal antibody, clone 1D7. Western Blot analysis of SMURF1 expression in HeLa.
Western Blotting SMURF1 monoclonal antibody, clone 1D7. Western Blot analysis of SMURF1 expression in HepG2.
Western Blotting Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody, clone 1D7. Lanes Lane 1: SMURF1 transfected lysate (83.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.18 kDa).
Immunoprecipitation Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with SMURF1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged SMURF1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_020429   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9HCE7 |
| Application: | Monoclonal Anti-SMURF1 antibody produced in mouse has been used in Western Blotting. |
| Biochem/physiol Actions: | SMAD specific E3 ubiquitin protein ligase 1 (SMURF1) acts as a negative regulator of transforming growth factor β (TGFβ) pathway. It has a role in the nuclear export of mothers against decapentaplegic homolog 7 (SMAD7) and the degradation of SMAD4. During epithelial-mesenchymal transition, SMURF1 dissolves tight junctions in cells. This protein also has a role in cell migration and in the bone morphogenetic protein pathway. SMURF1 has been associated with hepatocellular carcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SMAD specific E3 ubiquitin protein ligase 1 (SMURF1) is an E3 ubiquitin ligase and the gene encoding it is localized on human chromosome 7q22.1. |
| Immunogen: | SMURF1 (NP_065162, 165 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence DSGPGRPLSCFMEEPAPYTDSTGAA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |