Monoclonal Anti-TRIB3 antibody produced in mouse
SIGMA/WH0057761M3 - clone 1H2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-C20orf97; Anti-NIPK; Anti-SINK; Anti-SKIP3; Anti-TRB3; Anti-tribbles homolog 3 (Drosophila)
MDL Number: MFCD08705525
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TRIB3 on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of TRIB3 expression in transfected 293T cell line by TRIB3 monoclonal antibody, clone 1H2. Lanes Lane 1: TRIB3 transfected lysate (39.6 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of TRIB3 over-expressed 293 cell line, cotransfected with TRIB3 Validated Chimera RNAi. Blot probed with TRIB3 monoclonal antibody (M03) clone 1H2. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (35.53 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC027484   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96RU7 |
| General description: | The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. (provided by RefSeq) |
| Immunogen: | TRIB3 (AAH27484, 56 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PDRATAVATASRLGPYVLLEPEEGG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |