Monoclonal Anti-POLD4 antibody produced in mouse
SIGMA/WH0057804M1 - clone 2B11, ascites fluid
Synonym: Anti-POLDS; Anti-p12; Anti-polymerase (DNA-directed), delta 4
Product Type: Chemical
Western Blotting QC Western Blot detection against Immunogen (29.48 kDa).
ELISA Detection limit for recombinant GST tagged POLD4 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B11, monoclonal |
| conjugate | unconjugated |
| GenBank® accession no. | BC001334   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9HCU8 |
| Biochem/physiol Actions: | DNA polymerase (Pol) δ plays a vital role in DNA replication, DNA repair processes and genetic recombination. POLD4 stabilizes the Pol δ holoenzyme. In addition, it also facilitates pol δ-PCNA complex stabilization. Proteolysis of POLD4 in response to DNA damage leads to the consequent conversion of the holoenzyme pol δ4 to the heterotrimer pol δ3. Pol δ3 acts as an antimutator polymerase and is supposed to enhance surveillance against mutagenesis. Downregulated expression of the gene is associated with the genomic instability in lung cancer. |
| General description: | DNA polymerase δ 4 (POLD4), also known as p12, is the smallest subunit of DNA polymerase (Pol) δ. It is encoded by the gene mapped to human chromosome 11q13.2. |
| General description: | The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1 (MIM 174761), POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (Liu and Warbrick, 2006 [PubMed 16934752]). |
| Immunogen: | POLD4 (AAH01334, 1 a.a. ~ 34 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGRKRLITDSYPVVKRREGPAGHSK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |