Monoclonal Anti-JAM2 antibody produced in mouse
SIGMA/WH0058494M1 - clone 1G4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-C21orf43; Anti-JAMB; Anti-PRO245; Anti-VEJAM; Anti-junctional adhesion molecule 2
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to JAM2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1 μg/mL]
Western Blotting JAM2 monoclonal antibody, clone 1G4. Western Blot analysis of JAM2 expression in HL-60.
Western Blotting Western Blot analysis of JAM2 expression in transfected 293T cell line by JAM2 monoclonal antibody, clone 1G4. Lanes Lane 1: JAM2 transfected lysate (Predicted MW: 33.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting JAM2 monoclonal antibody, clone 1G4. Western Blot analysis of JAM2 expression in human ovarian cancer.
Western Blotting QC Western Blot detection against Immunogen (55.44 kDa).
ELISA Detection limit for recombinant GST tagged JAM2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC017779   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P57087 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. (provided by RefSeq) |
| Immunogen: | JAM2 (AAH17779, 29 a.a. ~ 298 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FSAPKDQQVVTAVEYQEAILACKTP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |