Monoclonal Anti-SMOC1 antibody produced in mouse
SIGMA/WH0064093M3 - clone 8F10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-SPARC related modular calcium binding 1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to SMOC1 on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of SMOC1 expression in transfected 293T cell line by SMOC1 monoclonal antibody, clone 8F10. Lanes Lane 1: SMOC1 transfected lysate (48.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting SMOC1 monoclonal antibody, clone 8F10 Western Blot analysis of SMOC1 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (33.66 kDa).
ELISA Detection limit for recombinant GST tagged SMOC1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8F10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_022137   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9H4F8 |
| Biochem/physiol Actions: | SMOC1 plays an essential role in ocular and limb development. It participates in the regulation of cell-matrix interaction by binding to many cell-surface receptors, the extracellular matrix, growth factors, and cytokines. It also functions in bone morphogenetic proteins (BMP) signaling cascade. Mutation in SMOC1 causes a rare autosomal-recessive disorder, microphthalmia and limb anomalies (MLA). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SPARC related modular calcium binding 1 (SMOC1) is an extracellular glycoprotein belonging to the SPARC family. It is localized and secreted in bone marrow derived mesenchymal stem cells (BMSCs) stimulated with osteogenic medium (OSM). The protein is expressed in the developing optic stalk, ventral optic cup, and limbs of mouse embryos. It is composed of five domains, an N-terminal follistatin-like (FS) domain, two thyroglobulin-like (TY) domains similar to SMOC, and an extracellular calcium-binding (EC) domain. |
| Immunogen: | SMOC1 (NP_071420, 150 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SVQNKTPVCSGSVTDKPLSQGNSGR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |