Monoclonal Anti-NADK antibody produced in mouse
SIGMA/WH0065220M1 - clone 5F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ13052; Anti-NAD kinase; Anti-RP1283E3.6; Anti-dJ283E3.1
Product Type: Chemical
Western Blotting NADK monoclonal antibody, clone 5F4. Western Blot analysis of NADK expression in HepG2.
Western Blotting NADK monoclonal antibody, clone 5F4. Western Blot analysis of NADK expression in NIH/3T3.
Western Blotting NADK monoclonal antibody, clone 5F4. Western Blot analysis of NADK expression in Raw 264.7.
Western Blotting Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody, clone 5F4. Lanes Lane 1: NADK transfected lysate (49.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting NADK monoclonal antibody, clone 5F4 Western Blot analysis of NADK expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (74.8 kDa).
ELISA Detection limit for recombinant GST tagged NADK is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC001709   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95544 |
| General description: | NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM |
| Immunogen: | NADK (AAH01709, 1 a.a. ~ 446 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEMEQEKMTMNKELSPDAAAYCCSA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |