Monoclonal Anti-C22orf18 antibody produced in mouse
SIGMA/WH0079019M1 - clone 4C12-2C8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC861; Anti-PANE1; Anti-bK250D10.2; Anti-chromosome 22 open reading frame 18
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody, clone 4C12-2C8. Lanes Lane 1: CENPM transfected lysate (Predicted MW: 19.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting C22orf18 monoclonal antibody, clone 4C12-2C8 Western Blot analysis of C22orf18 expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (45.54 kDa).
Immunoprecipitation Immunoprecipitation of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with CENPM rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged C22orf18 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C12-2C8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC000705   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9NSP4 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM |
| Immunogen: | C22orf18 (AAH00705, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSVLRPLDKLPGLNTATILLVGTED |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |