Monoclonal Anti-NEK11 antibody produced in mouse
SIGMA/WH0079858M1 - clone 4E1-1F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ23495; Anti-NIMA (never in mitosis gene a)- related kinase 11
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody, clone 4E1-1F4. Lanes Lane 1: NEK11 transfected lysate (55.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting NEK11 monoclonal antibody, clone 4E1-1F4 Western Blot analysis of NEK11 expression in HL-60.
Western Blotting NEK11 monoclonal antibody, clone 4E1-1F4. Western Blot analysis of NEK11 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (78.54 kDa).
ELISA Detection limit for recombinant GST tagged NEK11 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E1-1F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC028587   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8NG66 |
| General description: | This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described |
| Immunogen: | NEK11 (AAH28587, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLKFQEAAKCVSGSTAISTYPKTLI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |