Monoclonal Anti-COASY antibody produced in mouse
SIGMA/WH0080347M1 - clone 1H6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Coenzyme A synthase; Anti-DPCK; Anti-NBP; Anti-PPAT; Anti-UKR1; Anti-pOV2
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi. Blot probed with COASY monoclonal antibody, clone 1H6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody, clone 1H6. Lanes Lane 1: COASY transfected lysate (30.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting COASY monoclonal antibody, clone 1H6 Western Blot analysis of COASY expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (50.49 kDa).
ELISA Detection limit for recombinant GST tagged COASY is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006354   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13057 |
| General description: | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM |
| Immunogen: | COASY (AAH06354, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLGNLLRPPYERPELPTCLYVIGLT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |