Monoclonal Anti-POLR1B antibody produced in mouse
SIGMA/WH0084172M10 - clone 4H6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ10816; Anti-FLJ21921; Anti-MGC131780; Anti-RPA135; Anti-Rpo12; Anti-polymerase (RNA) I polypeptide B, 128kDa
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to POLR1B on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of POLR1B expression in transfected 293T cell line by POLR1B monoclonal antibody, clone 4H6. Lanes Lane 1: POLR1B transfected lysate (Predicted MW: 128.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged POLR1B is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_019014   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9H9Y6 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM |
| Immunogen: | POLR1B (NP_061887, 963 a.a. ~ 1072 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GEMLKAAGYNFYGTERLYSGISGLE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |