Monoclonal Anti-USP48 antibody produced in mouse
SIGMA/WH0084196M1 - clone 3G4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp762M1713; Anti-MGC14879; Anti-RAP1GA1; Anti-USP31; Anti-ubiquitin specific protease 48
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to USP48 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 μg/mL]
Western Blotting USP48 monoclonal antibody, clone 3G4 Western Blot analysis of USP48 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged USP48 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_032236   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q86UV5 |
| Biochem/physiol Actions: | Ubiquitin specific peptidase 48 (USP48) is a deubiquitinylating enzyme, that catalyzes the removal of ubiquitin (Ub) moieties from proteins. Inhibition of USP48 by dopamine D3 receptor (D3R) enhances Na+-H+ exchanger (NHE)-3 degradation, which is essential for regulation of blood pressure and sodium balance. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. (provided by RefSeq) |
| Immunogen: | USP48 (NP_115612, 110 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence NLELRQALYLCPSTCSDYMLGDGIQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |