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Monoclonal Anti-CAMKK1 antibody produced in mouse

SIGMA/WH0084254M1 - clone 1F6, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CAMKKA; Anti-DKFZp761M0423; Anti-MGC34095; Anti-calcium/calmodulin-dependent protein kinase kinase 1, alpha

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-WH0084254M1-100UG 100 µg
 $406.00
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Immunohistochemistry Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1 μg/mL]
Enhanced Validation-RNAi Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi. Blot probed with CAMKK1 monoclonal antibody (M01) clone 1F6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody, clone 1F6. Lanes Lane 1: CAMKK1 transfected lysate (57.31 kDa). Lane 2: Non-transfected lysate.
Western Blotting CAMKK1 monoclonal antibody, clone 1F6 Western Blot analysis of CAMKK1 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (82.94 kDa).
ELISA Detection limit for recombinant GST tagged CAMKK1 is approximately 1 ng/mL as a capture antibody.

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone 1F6, monoclonal
conjugate unconjugated
form buffered aqueous solution
GenBank accession no. BC031647 
isotype IgG1κ
Quality Level 100 
shipped in dry ice
species reactivity human
storage temp. −20°C
target post-translational modification unmodified
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: suitable
  western blot: 1-5 μg/mL
UniProt accession no. Q8N5S9 
Biochem/physiol Actions: Calcium/calmodulin-dependent protein kinase 1 (CAMKK1) participates in the calcium/calmodulin-dependent (CaM) kinase cascade. It phosphorylates CAMK1 at threonine 177. Functionally, the members of the CAMKKs family are involved in neurite development, long-term memory (LTM), and axon elongation.
General description: Calcium/calmodulin-dependent protein kinase kinase 1 (CAMKK1) is a member of the serine/threonine-protein kinase family. The CAMKK1 gene is mapped to human chromosome 17p13.2. It is expressed in the brain, as well as in the thymus and spleen.
Immunogen: CAMKK1 (AAH31647, 1 a.a. ~ 520 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEGPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLV
Legal Information: GenBank is a registered trademark of United States Department of Health and Human Services
Physical form: Solution in phosphate buffered saline, pH 7.4
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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