Monoclonal Anti-VPS25 antibody produced in mouse
SIGMA/WH0084313M1 - clone 2E5-2B9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-hypothetical protein MGC10540
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to VPS25 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting MGC10540 monoclonal antibody, clone 2E5-2B9 Western Blot analysis of MGC10540 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (45.1 kDa).
ELISA Detection limit for recombinant GST tagged VPS25 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E5-2B9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006282   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9BRG1 |
| General description: | VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM |
| Immunogen: | MGC10540 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAMSFEWPWQYRFPPFFTLQPNVDT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |