Monoclonal Anti-DOT1L antibody produced in mouse
SIGMA/WH0084444M1 - clone 6A6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DOT1; Anti-DOT1-like, histone H3 methyltransferase (S. cerevisiae); Anti-KIAA1814
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to DOT1L on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting DOT1L monoclonal antibody, clone 6A6. Western Blot analysis of DOT1L expression in LNCaP.
Western Blotting QC Western Blot detection against Immunogen (37.4 kDa).
ELISA Detection limit for recombinant GST tagged DOT1L is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6A6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_032482   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8TEK3 |
| Biochem/physiol Actions: | Disruptor of telomeric silencing 1 (DOT1) like histone lysine methyltransferase (DOT1L), is a nucleosomal enzyme that catalyzes the intra-nucleosomal methylation of H3 at lysine-79, a crucial step involved in the regulation of cell cycle, transcriptional regulation, and DNA damage response. Mammalian DOT1 gene plays an essential role in embryogenesis, hematopoiesis, cardiac function, and development of leukemia. Thus, DOT1L can act as a potential target for therapeutic treatment of the same. Elevated DOT1L enzymatic activity leads to MLL (mixed-lineage leukemia). Therefore, inhibition of DOT1L by SYC-522 in combination with DNA-damaging chemotherapy is considered as a potential treatment for MLL. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Disruptor of telomeric silencing 1 (DOT1) like histone lysine methyltransferase (DOT1L) is encoded by the gene mapped to human chromosome 19p13.3. |
| Immunogen: | DOT1L (NP_115871, 3 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EKLELRLKSPVGAEPAVYPWPLPVY |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |