Monoclonal Anti-TRIM63 antibody produced in mouse
SIGMA/WH0084676M1 - clone 6G6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ32380; Anti-IRF; Anti-MURF1; Anti-MURF2; Anti-RNF28; Anti-SMRZ; Anti-tripartite motif-containing 63
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TRIM63 on HeLa cell. [antibody concentration 10 μg/mL]
Enhanced Validation-RNAi Western blot analysis of TRIM63 over-expressed 293 cell line, cotransfected with TRIM63 Validated Chimera RNAi. Blot probed with TRIM63 monoclonal antibody, clone 6G6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody, clone 6G6. Lanes Lane 1: TRIM63 transfected lysate (40.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged TRIM63 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6G6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_032588   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q969Q1 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin′s N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. (provided by RefSeq) |
| Immunogen: | TRIM63 (NP_115977, 254 a.a. ~ 352 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence DKSTKLVETAIQSLDEPGGATFLLT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |