Monoclonal Anti-COG7 antibody produced in mouse
SIGMA/WH0091949M1 - clone 3G4-1B3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CDG2E; Anti-component of oligomeric golgi complex 7
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to COG7 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of COG7 expression in transfected 293T cell line by COG7 monoclonal antibody, clone 3G4-1B3. Lanes Lane 1: COG7 transfected lysate (65 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (110.44 kDa).
ELISA Detection limit for recombinant GST tagged COG7 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G4-1B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC037563   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P83436 |
| General description: | Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM |
| Immunogen: | COG7 (AAH37563, 1 a.a. ~ 770 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDFSKFLADDFDVKEWINAAFRAGS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |