Monoclonal Anti-PPP1R14A antibody produced in mouse
SIGMA/WH0094274M1 - clone 3A7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CPI17; Anti-PPP1INL; Anti-protein phosphatase 1, regulatory (inhibitor) subunit 14A
Product Type: Chemical
Western Blotting Western Blot analysis of PPP1R14A expression in transfected 293T cell line by PPP1R14A monoclonal antibody, clone 3A7. Lanes Lane 1: PPP1R14A transfected lysate (16.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (41.91 kDa).
ELISA Detection limit for recombinant GST tagged PPP1R14A is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC021089   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96A00 |
| General description: | PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM |
| Immunogen: | PPP1R14A (AAH21089, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAQRLGKRVLSKLQSPSRARGPGG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |