Monoclonal Anti-UHRF2 antibody produced in mouse
SIGMA/WH0115426M1 - clone 3A11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp434B0920; Anti-DKFZp686G0837; Anti-MGC33463; Anti-NIRF; Anti-RNF107; Anti-RP11472F14.2; Anti-URF2; Anti-ubiquitin-like, containing PHD and RING finger domains, 2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to UHRF2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of UHRF2 expression in transfected 293T cell line by UHRF2 monoclonal antibody, clone 3A11. Lanes Lane 1: UHRF2 transfected lysate (56.1 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of UHRF2 over-expressed 293 cell line, cotransfected with UHRF2 Validated Chimera RNAi. Blot probed with UHRF2 monoclonal antibody, clone 3A11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_152896   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96PU4 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. (provided by RefSeq) |
| Immunogen: | UHRF2 (NP_690856, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PGTSTQIEAKPCSNSPPKVKKAPRV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |