Monoclonal Anti-CALR3 antibody produced in mouse
SIGMA/WH0125972M1 - clone 4E3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CRT2; Anti-FLJ25355; Anti-MGC26577; Anti-calreticulin 3
Product Type: Chemical
Western Blotting Western Blot analysis of CALR3 expression in transfected 293T cell line by CALR3 monoclonal antibody, clone 4E3. Lanes Lane 1: CALR3 transfected lysate (45 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of CALR3 over-expressed 293 cell line, cotransfected with CALR3 Validated Chimera RNAi. Blot probed with CALR3 monoclonal antibody, clone 4E3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (65.78 kDa).
ELISA Detection limit for recombinant GST tagged CALR3 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC014595   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96L12 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Calreticulins, such as CALR3, are Ca(2+)-binding chaperones localized mainly in the endoplasmic/sarcoplasmic reticulum Persson et al. (2002) [PubMed 12384296].[supplied by OMIM |
| Immunogen: | CALR3 (AAH14595, 21 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VYFQEEFLDGEHWRNRWLQSTNDSR |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |