Monoclonal Anti-SH3D19 antibody produced in mouse
SIGMA/WH0152503M1 - clone 5C7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EVE1; Anti-MGC105136; Anti-MGC118910; Anti-SH3 domain protein D19
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SH3D19 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SH3D19 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SH3D19 monoclonal antibody, clone 5C7. Western Blot analysis of SH3D19 expression in Raw 264.7.
Western Blotting SH3D19 monoclonal antibody, clone 5C7 Western Blot analysis of SH3D19 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5C7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_001009555   |
| isotype | IgG3κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q5HYK7 |
| Biochem/physiol Actions: | SH3 domain containing 19 (SH3D19) inhibits processes induced by Ras oncoprotein. It binds to a protein called EEN (extra eleven-nineteen leukemia fusion gene protein), part of the endophilin family, and modulates the activity of ADAMs (a disintegrin and metalloproteases). |
| General description: | SH3 domain containing 19 (SH3D19) is a cytoplasmic protein and the gene encoding it is localized on human chromosome 4q31.3. |
| Immunogen: | SH3D19 (NP_001009555, 94 a.a. ~ 203 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PVTKPELPKKPNPGLIRSVNPEIPG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |