Monoclonal Anti-C9orf96 antibody produced in mouse
SIGMA/WH0169436M3 - clone 2B5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC43306; Anti-RP11244N20.8; Anti-chromosome 9 open reading frame 96
Product Type: Chemical
Western Blotting Western Blot analysis of C9orf96 expression in transfected 293T cell line by C9orf96 monoclonal antibody, clone 2B5. Lanes Lane 1: C9orf96 transfected lysate (75.5 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of C9orf96 over-expressed 293 cell line, cotransfected with C9orf96 Validated Chimera RNAi. Blot probed with C9orf96 monoclonal antibody, clone 2B5. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged C9orf96 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_153710   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL |
| General description: | Chromosome 9 open reading frame 96 (C9orf96) which is also known as serine/threonine kinase-like domain containing 1, is catalytically inactive due to its inactive protein kinase domain. |
| Immunogen: | C9orf96 (NP_714921, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MLGPGSNRRRPTQGERGPGSPGEPM |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |