Monoclonal Anti-IL27 antibody produced in mouse
SIGMA/WH0246778M1 - clone 3F12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IL27; Anti-IL27p28; Anti-IL30; Anti-MGC71873; Anti-interleukin 27; Anti-p28
MDL Number: MFCD02262986
Product Type: Chemical
Western Blotting IL27 monoclonal antibody, clone 3F12 Western Blot analysis of IL27 expression in HeLa.
Western Blotting Western Blot analysis of IL27 expression in transfected 293T cell line by IL27 monoclonal antibody, clone 3F12. Lanes Lane 1: IL27 transfected lysate (27.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (33.11 kDa).
Immunoprecipitation Immunoprecipitation of IL27 transfected lysate using anti-IL27 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with IL27 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_145659   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8NEV9 |
| Biochem/physiol Actions: | Interleukin-27 (IL27) has a role in immune responses. It aids in the secretion of interferon-γ and the activation of natural killer cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). (provided by RefSeq) |
| Immunogen: | IL27 (NP_663634, 177 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RKGLLPGALGSALQGPAQVSWPQLL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |