Monoclonal Anti-NEIL2 antibody produced in mouse
SIGMA/WH0252969M1 - clone 1B7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FLJ31644; Anti-MGC2832; Anti-MGC4505; Anti-NEH2; Anti-nei like 2 (E. coli)
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of NEIL2 over-expressed 293 cell line, cotransfected with NEIL2 Validated Chimera RNAi. Blot probed with NEIL2 monoclonal antibody, clone 1B7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of NEIL2 expression in transfected 293T cell line by NEIL2 monoclonal antibody, clone 1B7. Lanes Lane 1: NEIL2 transfected lysate (36.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (62.26 kDa).
ELISA Detection limit for recombinant GST tagged NEIL2 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC013964   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q969S2 |
| Biochem/physiol Actions: | Nei like DNA glycosylase 2 (NEIL2) cleaves base lesions caused due to oxidative damage. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])[supplied by OMIM |
| Immunogen: | NEIL2 (AAH13964, 1 a.a. ~ 332 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPEGPLVRKFHHLVSPFVGQQVVKT |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |