Monoclonal Anti-IBRDC2 antibody produced in mouse
SIGMA/WH0255488M2 - clone 4F1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-IBR domain containing 2; Anti-KIAA0161; Anti-MGC71786; Anti-bA528A10.3; Anti-p53RFP
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to IBRDC2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.2 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting IBRDC2 monoclonal antibody, clone 4F1 Western Blot analysis of IBRDC2 expression in IMR-32.
Western Blotting Western Blot analysis of RNF144B expression in transfected 293T cell line by IBRDC2 monoclonal antibody, clone 4F1. Lanes Lane 1: RNF144B transfected lysate (33.6 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_182757   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q7Z419 |
| Biochem/physiol Actions: | IBR domain containing 2 (IBRDC2) modulates the expression of the pro-apoptotic protein, Bax. It has a regulatory role in epithelial homeostasis and is involved in the stimulation of p53-dependent apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IBR domain containing 2 (IBRDC2) is also known as ring finger protein 144B (RNF144B). It is an E3 ubiquitin ligase which is expressed in the ovaries, testis and spleen. The 303 amino acid protein is localized to the mitochondria, Golgi apparatus, cell membrane and cytoplasm. The gene encoding it is located on human chromosome 6p22.3. |
| Immunogen: | IBRDC2 (NP_877434, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGSAGRLHYLAMTAENPTPGDLAPA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |