IROA TruQuant Yeast Extract Workflow Kit
SIGMA/WORKFLOW - Supplied by IROA Technologies
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change
The IROA TruQuant IQQ workflow is based on a well-characterized, long-term reference standard (D = LTRS) and a carefully matched internal standard (B = IS) to provide instrument and process QA/QC, as well as validated compound identification and quantitation.
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | TruQuant IQQ is a high-quality quantitation system for making simultaneous accurate biological measurements on several hundred biochemicals in small quantities of biological samples. This is achieved by (1) spiking a complex Internal Standard (WORKFLOW-A) into a biological sample to a) quantify all the biochemicals in the sample relative to their counterparts in the Internal Standard, b) suppression-correct each compound and c) normalize sample to sample variances; and (2) injecting the same well characterized Long-Term Reference Standard (WORKFLOW-B) to create a daily retention time (RT) library of all compounds to be found in the Internal Standard for reproducible ID, and to measure day-to-day (QA/QC) to assure reproducible instrument performance. The system is completely automated using IROA ClusterFinder™ software. IROA TruQuant IQQ Workflow Kit contains the materials and tools for the analysis of 90 experimental samples. The kit is intended to be used for mass spectrometry metabolomics applications. |
| Features and Benefits: | Identification: Confidence in identifying common and new metabolites Quantification: Comprehensive correction of analytical and biological variability for all analytes Quality Control: Convenient and automated tools for assuring reproducibility within/across studies • Simultaneous metabolite identification, quantitation and platform QC • Accurate compound formula ID for MS alone; complete ID with addition of SWATH, or IM • IROA patterns discriminate peaks of biological origin from artefactual peaks allowing the removal of false data • IROA-based fragments have the IROA ratio pattern of their parent peaks and can be identified as such using the “peak correlation” ClusterFinder™ module • Retention Index within the sample allows “unknowns” to be tracked with precision • Suppression-corrected measurements for significantly better quantitation • Normalizes (suppression-corrected) experimental samples to overcome day-to-day, instrument-to-instrument variances • ClusterFinder™ software solution builds libraries, IDs/quantitates compounds and normalizes data • Ensures high level QC for accurate and reproducible results |
| Legal Information: | ClusterFinder is a trademark of IROA Technologies LLC |
| Other Notes: | ClusterFinder™ software package is distributed with and is tailored to work with the IROA TruQuant IQQ kit. A user manual and video instructions are provided. To download the software package, click here - ClusterFinder software.  Link to the ClusterFinder™ Videos below: • IROA Peaks Basics • CF Process Overview • CF Initiation • CF New Project & Add Data • CF Unbiased Search for IROA peaks • CF Libraries & Methods • CF BinTree - bins are displayed as a tree • CF Curation 1: Untargeted - quality of the peaks • CF Curation 2: Cascade To access the MS Calculator Cascade Spreadsheet, follow the link below: MS Calculator  |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |