TransPlex® Whole Transcriptome Amplification Kit
SIGMA/WTA1 - DNA polymerase separate.
Product Type: Chemical
qPCR Primer Set. Differential expression of seven mRNAs (cDNAs) in human liver and UHR (Universal Human Reference) was measured by quantitative PCR. DC(t) values (UHR - Liver) were calculated for unamplified cDNA, prepared directly from RNA by reverse transcription, and amplification product prepared from 25 nanograms of total RNA with the Transplex WTA Kit. (Normalization of C(t)s for liver or brain was accomplished by subtracting the average DC(t) for all primer sets for a given tissue from each tissue-specific DC(t).) Uniformity between unamplified and amplified DC(t)s for individual mRNAs demonstrates maintenance of differential patterns of RNA expression in liver and UHR when using the Transplex WTA Kit. Universal Human Reference Total RNA is a product of Stratagene.
Kit Image
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | whole genome amplification: suitable |
| Application: | Suitable for use with downstream applications including: • qPCR • microarray analysis • cloning |
| Application: | TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA. |
| Features and Benefits: | • Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time • Only 5 ng of starting material required to produce a highly representative library from total RNA • Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples |
| General description: | TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses. |
| Legal Information: | TransPlex is a registered trademark of Rubicon Genomics, Inc. |
| Principle: | The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Water, Nuclease-Free Water, for Molecular Biology; Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent |