Complete Whole Transcriptome Amplification Kit
SIGMA/WTA2 - DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias
Synonym: transcriptome amplification kit
Product Type: Chemical
Kit Image
Complete Whole Transcriptome Amplification Kit, Cat. No. WTA2 : The TransPlex WTA process has two steps: 1) library synthesis and 2) library amplification. Synthesize a linear cDNA library by adding sample RNA to reverse transcriptase and WTA primers comprised of a quasi-random 3′ end and a universal 5′ end (second step). Universal-primer PCR amplifies the cDNA library, producing a faithfully amplified whole transcriptome. Annealed primers are extended by WTA polymerase, acting as new templates for primer annealing and extension. This process creates an OmniPlex® library of overlapping 100–1 000 bp amplicons, flanked by a universal end sequence. The final cDNA library is a result of linear amplification. Re-amplification of the cDNA library is possible.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | whole genome amplification: suitable |
| Application: | Complete Whole Transcriptome Amplification Kit is used for the following applications: • To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing. • Reverse transcription and cDNA amplification • For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles • Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis) • Amplification of RNA andssDNA viruses for Viral metagenomics from low-input clinical samples |
| Application: | Suitable for use with downstream applications including: • qPCR • microarray analysis • cloning |
| Biochem/physiol Actions: | The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product. |
| Features and Benefits: | • Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required • Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling • Contains all needed components for cDNA amplification • Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias • Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism |
| General description: | WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 41121800 |
| Components | Library Synthesis Enzyme; Library Synthesis Solution; Amplification Mix; Library Synthesis Buffer; Water, Nuclease-Free Water, for Molecular Biology; Amplification Enzyme; Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent |