Anti-XRCC1 antibody produced in rabbit
SIGMA/X0629 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-X-ray repair complementing defective repair in Chinese hamster cells 1
MDL Number: MFCD01865396
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Base excision by thymine DNA glycosylase mediates DNA-directed cytotoxicity of 5-fluorouracil. By: Kunz, C., Focke, F., et al. in PLoS Biol, 2009. PubMed ID: 19402749 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pbio.1000091), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence MCF7 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Anti-XRCC1 antibody produced in Rabbit (Cat. No. X0629) followed by 1:500 Anti-Rabbit IgG-Cy3.
Western Blotting A431 cell lysates were separated on SDS-PAGE and probed with Anti-XRCC1 antibody produced in Rabbit (Cat. No. X0629). The antibody was developed using 1:35,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. 1 μg/mL antibody + inhibition with immunizing peptide 5. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 85 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton |
| western blot: 0.5-1 μg/mL using A431 cell lysates | |
| UniProt accession no. | P18887  |
| Application: | Anti-XRCC1 antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • indirect immunofluorescence and telomere FISH (TEL-FISH) • protein detection • western analysis |
| Biochem/physiol Actions: | X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene. |
| Immunogen: | synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |