Anti-XPC (C-terminal) antibody produced in rabbit
SIGMA/X1129 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Xeroderma pigmentosum, complementary group C; Anti-XP3; Anti-XPCC
MDL Number: MFCD09265382
Product Type: Chemical
Immunofluorescence Nuclear localization of XPC in MCF7 cells. Cells were stained with 10 mg/mL of Rabbit Anti-XPC (C-Terminal) (Cat. No. X 1129) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (Panel A) and with DAPI (Panel B)
Immunoblotting MCF-7 Lysate was separated on SDS-PAGE, probed with Rabbit Anti-XPC C-Terminal (Cat. No. X1129) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) using a chemiluminescence substrate. Lanes 1. Antibody 2.0 μg/mL 2. Antibody 1.0 μg/mL 3. Antibody 0.5 μg/mL 4. Antibody 0.25 μg/mL 6. Antibody 2.0 μg/mL + 5 μg/mL XPC C-Terminal immunizing peptide 7. Antibody 1.0 μg/mL + 5 μg/mL XPC C-Terminal immunizing peptide 8. Antibody 0.5 μg/mL + 5 μg/mL XPC C-Terminal immunizing peptide 9. Antibody 0.5 μg/mL + 5 μg/mL non-relevant peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 120 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton |
| western blot: 0.5-1 μg/mL using MCF-7 cell lysates | |
| UniProt accession no. | Q01831  |
| Application: | Anti-XPC (C-terminal) antibody produced in rabbit has been used in indirect immunofluorescence and western blotting |
| Biochem/physiol Actions: | Defective XPC gene causes photosensitivity syndrome called xeroderma pigmentosum (XP), which is characterized by a very high incidence of light-induced skin cancer. |
| Biochem/physiol Actions: | DNA damage recognition and repair factor, XPC complex subunit xeroderma pigmentosum group C (XPC) has a role in global genome nucleotide excision repair pathway. As part of the XPC complex, this protein binds to DNA sites having many lesions. Single nucleotide polymorphisms in the XPC gene have been linked to various cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DNA damage recognition and repair factor, XPC complex subunit xeroderma pigmentosum group C (XPC) is a DNA damage recognition factor. The protein consists of 940 amino acids. The gene encoding XPC is localized on human chromosome 3p25.1 and consists of 18 exons. |
| General description: | XPC exists in vivo as a heterotrimeric complex with one of the two mammalian homologs of S. cerevisiae Rad23 (HR23A or HR23B) and centrin 2. |
| Immunogen: | synthetic peptide corresponding to amino acids 922-940 of human XPC, conjugated to KLH via an N-terminal added cysteine residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |