Anti-XPA (C-terminal) antibody produced in rabbit
SIGMA/X1254 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-XP1; Anti-XPAC; Anti-Xeroderma pigmentosum, complementary group A
MDL Number: MFCD09265380
Product Type: Chemical
Western Blotting Whole extract of Rat-1 cells were separated on SDS-PAGE and probed with Anti-XPA (C-terminal) antibody produced in Rabbit (Cat. No. X1254). The antibody was developed using 1:50,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:500 antibody + inhibition with immunizing peptide
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 35 kDa (doublet) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:1,000-1:2,000 using Jurkat cell lysates |
| western blot: 1:250-1:500 using Rat1 cell lysates | |
| UniProt accession no. | P23025  |
| Application: | Anti-XPA (C-terminal) antibody produced in rabbit has been used in immunolabeling and immunoblotting. |
| Biochem/physiol Actions: | Xeroderma pigmentosum group A-complementing protein (XPA) interacts with nucleotide excision repair (NER) subunits, such as replication protein A (RPA), excision repair complementing 1 protein (ERCC1), and transcription factor II (TFIIH). However, it is widely accepted that the Xeroderma pigmentosum group C-complementing protein (XPC)-human Rad23 homolog (hHR23B) complex recognizes the DNA damage-induced helical distortion. After this, the transcription factor IIH (TFIIH), XPA (possibly in its homodimeric form), and replication protein A (RPA) arrive sequentially at the site of damage. XPA interacts directly with DNA via the zinc finger motif. RPA coordinates with XPA in the positioning in the nucleotide excision repair (NER) bubble. Defects in the excision repair leads to photosensitivity syndrome called xeroderma pigmentosum (XP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Xeroderma pigmentosum group A-complementing protein (XPA) or DNA repair protein complementing XP-A cells human excision repair protein. XPA encodes a hydrophilic metalloprotein. It displays a C4 zinc finger motif, a central globular domain and has disordered regions in the N and C-terminus. The gene XPA is localized in human chromosome 9q22.33. |
| Immunogen: | synthetic peptide corresponding to amino acids 257-273 of human XPA, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences by one amino acid. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-XPA (C-terminal) specifically recognizes human XPA. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |