Anti-XPG (C-terminal) antibody produced in rabbit
SIGMA/X1629 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-COFS3; Anti-ERCC5; Anti-ERCM2; Anti-UVDR; Anti-XPGC
Product Type: Chemical
Immunocytochemistry Nuclear localization of XPG in HEK-293T transfected with XPG-eGFP expression plasmid. A. Cell were stained with Rabbit Anti-XPG (C-terminal) (Cat. No. X1629) at 2 μg/mL and developed with Goat Anti-Rabbit IgG, Cy3 conjugated B. DAPI staining C. eGFP signal
Immunoblotting Whole cell lysates of COS7 cells transfected with XPG-eGFP expression plasmid were separated on SDS-PAGE and probed with Rabbit Anti-XPG (C-terminal) (Cat. No. X1629) at 1 μg/mL. The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 1 μg/mL 2: Antibody 1 μg/mL + 20 μg/mL XPG immunizing peptide (Human, 1120-1137).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2-4 μg/mL using HEK-293T cells expressing recombinant XPG fusion protein, fixed with paraformaldehyde-Triton |
| western blot: 1-2 μg/mL using whole cell lysates of COS7 cells expressing recombinant XPG fusion protein | |
| UniProt accession no. | P28715   |
| Application: | Anti-XPG (C-terminal) antibody produced in rabbit has been used in: • staining cells for xeroderma pigmentosum group G (XPG) detection • immunoblotting • immunofluorescence |
| Biochem/physiol Actions: | Xeroderma pigmentosum group G (XPG) helps to maintain genomic stability. It also participates in RNA transcription by interacting with TFIIH, RNA polymerase II and Gadd45a. XPG also participates in nucleotide excision repair (NER) pathway. Mutations in the XPG gene cause the Cockayne syndrome which is characterized by severe growth defects, mental retardation and cachexia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-XPG (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to amino acids 1120-1137 of human XPG, conjugated to KLH via an N-terminal added cysteine residue. Xeroderma pigmentosum group G (XPG) is a DNA repair gene, that is mapped to human chromosome13q33. It is a member of the FEN-1 family of structure-specific nucleases and contains two highly conserved nuclease motifs known as the N and I regions separated by a large insertion. This gene spans around 69kb in length and has15 exons. It is highly polymorphic. |
| Immunogen: | synthetic peptide corresponding to amino acids 1120-1137 of human XPG, conjugated to KLH via an N-terminal added cysteine residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |