Xanthine Oxidase from bovine milk
SIGMA/X1875 - Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein
Synonym: XOD; Xanthine:oxygen oxidoreductase
CAS Number: 9002-17-9
EC Number: 232-657-6
MDL Number: MFCD00082145
Product Type: Chemical
| foreign activity | uricase ≤0.5% |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥0.4 units/mg protein |
| storage temp. | 2-8°C |
| type | Grade I |
| Analysis Note: | Protein determined by biuret |
| Application: | Xanthine Oxidase from bovine milk has been used: • in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5- • in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity • as a standard to determine XO activity • as a standard to test the synergistic effect of docosahexaenoic acid (DHA) |
| Biochem/physiol Actions: | Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme. |
| Biochem/physiol Actions: | Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. |
| Biochem/physiol Actions: | Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c. |
| General description: | Formerly E.C. 1.1.3.22 |
| General description: | Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites. |
| Packaging: | 5, 25 units in serum bottle |
| Physical form: | Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate |
| Unit Definition: | One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.4 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.17.3.2. |
| UNSPSC | 12352204 |