Xanthine Oxidase microbial
SIGMA/X2252 - lyophilized powder, ≥7 units/mg solid
Synonym: XOD; Xanthine:oxygen oxidoreductase
CAS Number: 9002-17-9
EC Number: 232-657-6
MDL Number: MFCD00082145
Product Type: Chemical
Figure 2. Stability (liquid form); in 50 mM buffer solution, 4 °C, pH 6.8.
Figure 3. pH Activity; 37 °C in 4-min. reaction, in 50 mM buffer solution ●: pH 6.0–8.2 K-phosphate ○: pH 7.5–8.5 Tris-HCl
Figure 4. Thermal activity; in 50 mM Tris-HCl buffer, pH 8.0
Figure 5. pH Stability; 25 °C, 15-hr treatment with 50 mM buffer solution: ●: pH 4.0–6.0, acetate ○: pH 6.0–8.0 K-phosphate ■: pH 8.0–9.0 Tris-HCl □: pH 9.0–10.0 Na-K carbonate
Figure 6. Thermal stability; 30-min. treatment with 50 mM Tris-HCl buffer, pH 8.0
| form | lyophilized powder |
| mol wt | ~160 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥7 units/mg solid |
| storage temp. | −20°C |
| UniProt accession no. | Q46799  |
| Q46800 |
|
| Q46801 |
| Application: | This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase (PNP-301) and uricase (UAO-201, UAO-211). |
| Biochem/physiol Actions: | Also contains proteolytic activity. |
| General description: | Allopurinol is a synthetic drug show to inhibit xanthine oxidase. |
| Packaging: | 25, 100 units in glass bottle |
| Physical form: | Lyophilized powder containing BSA and sodium glutamate as stabilizers |
| Physical properties: | Inhibitors: Reducing agents, Hg++, Ag+,MIA Optimum pH: 7.5 - 8.0 Optimum temperature: 65oC pH Stability: pH 6.5 - 9.0 (25oC, 15hr) Thermal stability: below 55oC (pH 8.0, 30min) |
| Unit Definition: | One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥7 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.17.3.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |