Xanthine Oxidase microbial
SIGMA/X2252 - lyophilized powder, ≥7 units/mg solid
Synonym: Schardingerenzyme; XO; hypoxanthine oxidase; XOD; Xanthine:oxygen oxidoreductase
CAS Number: 9002-17-9
EC Number: 232-657-6
MDL Number: MFCD00082145
Product Type: Chemical
Figure 2. Stability (liquid form); in 50 mM buffer solution, 4 °C, pH 6.8.
Figure 3. pH Activity; 37 °C in 4-min. reaction, in 50 mM buffer solution ●: pH 6.0–8.2 K-phosphate ○: pH 7.5–8.5 Tris-HCl
Figure 4. Thermal activity; in 50 mM Tris-HCl buffer, pH 8.0
Figure 5. pH Stability; 25 °C, 15-hr treatment with 50 mM buffer solution: ●: pH 4.0–6.0, acetate ○: pH 6.0–8.0 K-phosphate ■: pH 8.0–9.0 Tris-HCl □: pH 9.0–10.0 Na-K carbonate
Figure 6. Thermal stability; 30-min. treatment with 50 mM Tris-HCl buffer, pH 8.0
| biological source | microbial |
| color | brown |
| form | lyophilized powder |
| mol wt | ~160 kDa |
| optimum pH | 7.5-8.0 |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥7 units/mg solid |
| storage temp. | −20°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | Q46799  |
| Q46800 |
|
| Q46801 |
| Application: | Xanthine Oxidase microbial has been used: • to determine in vitro xanthine oxidase activity • as a source to generate superoxide anions during superoxide dismutase assay • as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction |
| Biochem/physiol Actions: | Also contains proteolytic activity. |
| Biochem/physiol Actions: | Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout. |
| General description: | Inhibitors: Reducing agents, Hg++, Ag+,MIA Optimum pH: 7.5 - 8.0 Optimum temperature: 65oC pH Stability: pH 6.5 - 9.0 (25oC, 15hr) Thermal stability: below 55oC (pH 8.0, 30min) |
| General description: | Research area: Cell Cycle Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit. |
| Other Notes: | One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate. |
| Packaging: | 25, 100 units in glass bottle |
| Physical form: | Lyophilized powder containing BSA and sodium glutamate as stabilizers |
| Preparation Note: | Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥7 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.17.3.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |