Xanthine Oxidase from bovine milk
SIGMA/X4500 - Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein
Synonym: Schardinger enzyme; XnOx; hypoxanthine oxidase; XOD; Xanthine:oxygen oxidoreductase
CAS Number: 9002-17-9
EC Number: 232-657-6
MDL Number: MFCD00082145
Product Type: Chemical
| biological source | bovine milk |
| color | faint brown to dark brown |
| foreign activity | uricase ≤0.1% |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥0.8 units/mg protein |
| storage temp. | 2-8°C |
| technique(s) | inhibition assay: suitable |
| type | Grade III |
| UniProt accession no. | A0A3Q1LW91  |
| A0A3Q1MM92 |
|
| A0A3S5ZPW7 |
| Analysis Note: | Protein determined by biuret |
| Application: | Xanthine Oxidase from bovine milk has been used: • as a source for superoxide generation • for the degradation of hypoxanthine • for the detection of reactive oxygen species (ROS) in lipid model by electron spin resonance (ESR) spin trap method • as a constituent of assay mixture in xanthine oxidase inhibition assay |
| Biochem/physiol Actions: | Xanthine Oxidase (XO)catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Xanthine oxidase activity is inhibited by folic acid. [7] and various other inhibitors including 3,4-Dihydroxy-5-nitrobenz |
| Biochem/physiol Actions: | Xanthine oxidase activity is inhibited by folic acid. |
| Biochem/physiol Actions: | Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. |
| General description: | Formerly E.C. 1.1.3.22 |
| General description: | Research area: Cell Cycle Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit. |
| Other Notes: | One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate. |
| Physical form: | Suspension in 2.3 M (NH4)2SO4, 10 mM sodium phosphate buffer, pH 7.8, containing 1 mM EDTA and 1 mM sodium salicylate |
| Preparation Note: | Chromatographically purified |
| Preparation Note: | Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.8 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.17.3.2. |
| UNSPSC | 12352204 |