Synonym: 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt; 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
CAS Number: 111072-31-2
Empirical Formula (Hill Notation): C22H16N7NaO13S2
Molecular Weight: 673.52
MDL Number: MFCD00083517
Linear Formula: C22H16N7NaO13S2
Product Type: Chemical
| application(s) |
detection |
| assay |
≥90% |
| detection method |
colorimetric |
| form |
powder |
| InChI |
1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1 |
| InChI key |
JACYMBNQPPWQML-UHFFFAOYSA-M |
| product line |
BioReagent |
| Quality Level |
200  |
| SMILES string |
[Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O |
| storage condition |
dry at room temperature |
| storage temp. |
2-8°C |
| technique(s) |
cell culture | mammalian: suitable |
| |
protein quantification: suitable |
| Application: |
XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported. |
| Application: |
XTT sodium salt has been used to assess cell viability. |
| Biochem/physiol Actions: |
XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed. |
| General description: |
XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction. |
| Packaging: |
100, 500 mg in poly bottle |
| Principle: |
In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product. |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥90% |
| Storage Temp. |
2-8°C |
| UNSPSC |
12352200 |
