Xanthine Oxidase from bovine milk
SIGMA/X4875 - Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein
Synonym: XOD; Xanthine:oxygen oxidoreductase
CAS Number: 9002-17-9
EC Number: 232-657-6
MDL Number: MFCD00082145
Product Type: Chemical
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| biological source | bovine milk |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥0.1 units/mg protein |
| storage temp. | 2-8°C |
| type | Grade IV |
| Analysis Note: | Protein determined by biuret |
| Application: | Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation. |
| Biochem/physiol Actions: | Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys. |
| Biochem/physiol Actions: | Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. |
| General description: | Formerly E.C. 1.1.3.22 |
| General description: | Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers. |
| Other Notes: | One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate. |
| Physical form: | Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.1 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.17.3.2. |
| UNSPSC | 12352204 |