REDExtract-N-Amp™ Plant PCR Kit
SIGMA/XNAPE - sufficient for 100 extractions, sufficient for 500 amplifications
Synonym: Plant direct PCR kit; Plant direct PCR kit with loading dye
Product Type: Chemical
PCR analysis of genomic DNA isolated from plant using Sigma′s Extract-N-Amp™ Plant PCR Kit.
The Extract-N-Amp™ Plant PCR Kit was used to extract and amplify genomic DNA from 0.5 cm leaf disks from various plant sources. The products were generated from a 30-cycle duplex reaction containing primers specific to plant chloroplast (upper band) and primers specific to a Cannabis sativa gene (lower band). MW ladder is 100, 200, 400, and 800 bp. Data provided by Andy Hopwood, Forensic Science Service, Birmingham, England.
The Extract-N-Amp™ Plant PCR Kit was used to extract and amplify genomic DNA from 0.5 cm leaf disks from various plant sources. The products were generated from a 30-cycle duplex reaction containing primers specific to plant chloroplast (upper band) and primers specific to a Cannabis sativa gene (lower band). MW ladder is 100, 200, 400, and 800 bp. Data provided by Andy Hopwood, Forensic Science Service, Birmingham, England.
Stability of Extract-N-Amp™ Plant extracts.
Eight disks were punched from a corn leaf, and DNA was extracted with the Extract-N-Amp™ Plant PCR Kit. Following extraction, two 4-μL aliquots from each sample were analyzed immediately by quantitative PCR with SYBR® Green detection on an ABI PRISM® 7700. DNA standards for quantitative PCR were purified DNA prepared from corn leaf tissue with the GenElute™ Plant Genomic DNA Miniprep Kit (Cat. No. G2N70). Half of the leaf extracts were stored at 4 °C (recommended storage conditions) and the other half at 37 °C (accelerated storage conditions). Quantitative PCR was repeated after 1, 3, and 6 months from extract at 4 °C, and after 1 week, 3 weeks, 6 weeks, and 6 months from extracts at 37 °C. Results for storage at 37 °C are shown. Results indicate that extract will be stable 6 months and probably several years at 4 °C. (data not shown)
Eight disks were punched from a corn leaf, and DNA was extracted with the Extract-N-Amp™ Plant PCR Kit. Following extraction, two 4-μL aliquots from each sample were analyzed immediately by quantitative PCR with SYBR® Green detection on an ABI PRISM® 7700. DNA standards for quantitative PCR were purified DNA prepared from corn leaf tissue with the GenElute™ Plant Genomic DNA Miniprep Kit (Cat. No. G2N70). Half of the leaf extracts were stored at 4 °C (recommended storage conditions) and the other half at 37 °C (accelerated storage conditions). Quantitative PCR was repeated after 1, 3, and 6 months from extract at 4 °C, and after 1 week, 3 weeks, 6 weeks, and 6 months from extracts at 37 °C. Results for storage at 37 °C are shown. Results indicate that extract will be stable 6 months and probably several years at 4 °C. (data not shown)
Representative image of product packaging.
Representative image of product packaging.
Representative image of product packaging.
| color | red |
| feature | dNTPs included |
| hotstart | |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| usage | sufficient for 100 extractions |
| sufficient for 500 amplifications | |
| sufficient for 500 reactions |
| Application: | REDExtract-N-Amp™ Plant PCR Kit has been used to extract genomic DNA from plants and to perform polymerase chain reaction. |
| Components: | Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix. |
| Features and Benefits: | • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes • No freezing, mechanical disruption, organic extraction, column purification or precipitation required • Specially formulated PCR ReadyMix for use with extract • Hot Start antibody for highly specific PCR amplification of genomic DNA • REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis • Compatible with high-throughput requirements for genetic analysis of plants • Extract stable at 4 °C for at least 6 months |
| General description: | The REDExtract-N-Amp™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis. |
| Legal Information: | Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/ext. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |