Extract-N-Amp™ Plant PCR Kit
SIGMA/XNAR - sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonym: Plant direct PCR kit
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| color | colorless |
| feature | hotstart |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for 1000 amplifications |
| sufficient for 1000 extractions |
| Application: | Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping. It has been used forDNA extraction from fresh mushrooms. |
| Application: | Extract-N-Amp™ Plant PCR Kit has been used for DNA extraction from fresh mushrooms. |
| Features and Benefits: | • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method. • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary • A specially formulated PCR ReadyMix is provided for use with the extracted DNA • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA • Suitable for high-throughput plant genetic analysis. • Lengthy enzymatic digestions are not required, saving time and effort. • The extracted DNA remains stable at 4 °C for atleast 6 months. |
| Features and Benefits: | • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes • A PCR ReadyMix™, specially formulated for amplification directly from extract • Hot Start antibody for highly specific PCR amplification of genomic DNA • Extract stable at 4 °C for at least 6 months |
| General description: | The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix™, especially formulated for amplification directly from extract. Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit. |
| General description: | The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues. |
| Legal Information: | Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody) |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/ext. |
| Other Notes: | The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106303 |