REDExtract-N-Amp™ Tissue PCR Kit
SIGMA/XNAT - sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included
Synonym: Tissue direct PCR kit; Tissue direct PCR kit with loading dye
EC Number: 254-457-8
Product Type: Chemical
PCR analysis of genomic DNA extracted from various samples using Sigma’s Extract-N-Amp™ Tissue PCR Kit.
The Extract-N-Amp Tissue PCR kit was used to extract and amplify genomic DNA from various sources. Genomic DNA was extracted from the samples using the appropriate protocol for each sample type as described in the Extract-N-Amp Tissue Technical Bulletin. In all cases, the extraction procedure was completed in less then 15 minutes. The extracted DNA was then amplified using the specially formulated hot start PCR mix, provided in the kit. The products generated are 1181 bp for the Interleukin 1 Beta gene in mouse and 1820 bp for the Carnitine palmitoyltransferase II in human. Markers are both PCR Marker (Cat. No. P9577).
The Extract-N-Amp Tissue PCR kit was used to extract and amplify genomic DNA from various sources. Genomic DNA was extracted from the samples using the appropriate protocol for each sample type as described in the Extract-N-Amp Tissue Technical Bulletin. In all cases, the extraction procedure was completed in less then 15 minutes. The extracted DNA was then amplified using the specially formulated hot start PCR mix, provided in the kit. The products generated are 1181 bp for the Interleukin 1 Beta gene in mouse and 1820 bp for the Carnitine palmitoyltransferase II in human. Markers are both PCR Marker (Cat. No. P9577).
Stability of DNA in mouse-tail extracts.
Mouse-tail samples were extracted according to the procedure in the Technical Bulletin for the Extract-N-Amp™ Tissue PCR Kit. The remaining mouse-tail tissue was removed from the samples for storage. 4 μL aliquots were analyzed immediately by quantitative PCR with SYBR® Green detection on an ABI Prism 7700. DNA standards for quantitative PCR were purified DNA prepared from mouse tails using the GenElute Mammalian Genomic DNA kit (Cat. No. G1N70) and stored as single use aliquots at –20 °C. The mouse-tail extracts were stored at 37 °C (accelerated storage). Quantitative PCR was repeated after 3 weeks, 5 weeks and 2 months from extracts at 37 °C. Results for storage at 37 °C are shown. These results suggest that extracts will be stable for at least 6 months at the recommended storage temperature of 4 °C.
Mouse-tail samples were extracted according to the procedure in the Technical Bulletin for the Extract-N-Amp™ Tissue PCR Kit. The remaining mouse-tail tissue was removed from the samples for storage. 4 μL aliquots were analyzed immediately by quantitative PCR with SYBR® Green detection on an ABI Prism 7700. DNA standards for quantitative PCR were purified DNA prepared from mouse tails using the GenElute Mammalian Genomic DNA kit (Cat. No. G1N70) and stored as single use aliquots at –20 °C. The mouse-tail extracts were stored at 37 °C (accelerated storage). Quantitative PCR was repeated after 3 weeks, 5 weeks and 2 months from extracts at 37 °C. Results for storage at 37 °C are shown. These results suggest that extracts will be stable for at least 6 months at the recommended storage temperature of 4 °C.
Representative image of product packaging.
Representative image of product packaging.
Representative image of product packaging.
| color | red |
| feature | dNTPs included |
| hotstart | |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| usage | sufficient for 10 reactions |
| sufficient for 100 reactions | |
| sufficient for 1000 reactions | |
| 10 reactions sufficient for 10 amplifications | |
| 10 reactions sufficient for 10 extractions | |
| 100 reactions sufficient for 100 amplifications | |
| 100 reactions sufficient for 100 extractions | |
| 1000 reactions sufficient for 1000 amplifications | |
| 1000 reactions sufficient for 1000 extractions |
| Application: | REDExtract-N-Amp™ tissue PCR kit has been used for DNA extraction and in polymerase chain reaction (PCR). |
| Components: | Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix. |
| Features and Benefits: | • Perfect for quick genomic DNA isolation for genotyping • Single-step extraction of genomic DNA for PCR in 15 minutes • PCR ReadyMix, specially formulated for amplification directly from extract • Hot Start antibody for highly specific PCR amplification of genomic DNA • Extract stable at 4oC for at least 6 months • REDExtract-N-Amp™ contains a tracking dye for convenient and direct loading of PCR reactions onto agarose gels for analysis |
| General description: | The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA. The kit includes a PCR ReadyMix™, specially formulated for direct PCR amplification from the extract. This formulation uses an antibody-based hot start, for specific amplification. The REDExtract-N-Amp Tissue PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis. |
| Legal Information: | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/ext. |
| Other Notes: | The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis. |
| Principle: | The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |