Yeast Nitrogen Base Without Amino Acids
SIGMA/Y0626 - Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements
Synonym: ynb without amino acids
Product Type: Chemical
| application(s) | food and beverages |
| form | powder |
| grade | for molecular biology |
| pH | 5.2-5.6(0.67% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Candida spp. |
| nonselective for Pichia spp. | |
| nonselective for Saccharomyces spp. | |
| nonselective for Zygosaccharomyces spp. | |
| yeasts | |
| technique(s) | microbe id | utilization test: suitable |
| Application: | Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required. |
| Application: | Yeast Nitrogen Base Without Amino Acids has been used: • in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterization • as a component of selective liquid media for uracil auxothrophic growth in yeast • to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions |
| Components: | Component, amount (μg/L unless indicated) Nitrogen Sources: Ammonium sulfate, 5.0 g/L Vitamins: Biotin, 2.0 Calcium pantothenate, 400 Folic acid, 2.0 Inositol, 2.0 mg/L Nicotinic acid, 400 p-Aminobenzoic acid, 200 Pyridoxine HCl, 400 Riboflavin, 200 Thiamine HCL, 400 Trace Elements: Boric acid, 500 Copper sulfate, 40 Potassium iodide, 100 Ferric chloride, 200 Manganese sulfate, 400 Sodium molybdate, 200 Zinc sulfate, 400 Salts: Potassium phosphate monobasic, 1.0 g/L Magnesium sulfate, 0.5 g/L Sodium chloride, 0.1 g/L Calcium chloride, 0.1 g/L |
| General description: | Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts. |
| General description: | Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome. |
| Preparation Note: | 1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water. 2. Warm if necessary to solubilize and sterilize by filtration. Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |