Yeast Synthetic Drop-out Medium Supplements
SIGMA/Y0750 - without leucine and tryptophan
MDL Number: MFCD01095679
Product Type: Chemical
application(s) | food and beverages |
form | powder |
Quality Level | 100 ![]() |
Application: | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
Components: | The supplements contain all of the following components except as indicated: Amino acids: All standard amino acids except for leucine and tryptophan. All the amino acids are present at a concentration of 76 mg/L. Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L). |
Other Notes: | Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids. |
Packaging: | 20 g in poly bottle |
Quantity: | 1.54 g will supplement 1 liter of medium. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12000000 |