Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal
SIGMA/Y0771 - purified from hybridoma cell culture, clone PYP-76
Synonym: Anti-COB1; Anti-YAP; Anti-YAP-1; Anti-YAP2; Anti-YAP65; Anti-YKI
Product Type: Chemical
Western Blotting Whole extract of HEK-293T cells expressing Human YAP1 and c-Abl were separated on SDS-PAGE and probed with Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal, Clone: PYP-76 (Cat. No. Y0771). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PYP-76, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgM |
| mol wt | antigen ~65 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 0.5-1 μg/mL using total cell extract of HEK-293T cells co-transfected with wt YAP and a constitutively active mutant of c-Abl (Δ1-81) | |
| UniProt accession no. | P46937  |
| Application: | Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal has been used in • immunoblotting • enzyme linked immunosorbent assay (ELISA) • immunofluorescence |
| Biochem/physiol Actions: | YAP1 has been shown to stabilize p73 by preventing Itch-mediated ubiquitination of p73. |
| Biochem/physiol Actions: | YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-phospho-YAP1 (pTyr357) (mouse IgM isotype) is derived from the hybridoma PYP-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phosphopeptide corresponding to a fragment containing (pTyr357) of human YAP1 conjugated to KLH. Yes-associated protein 1 (YAP1) is a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with the Src-family tyrosine kinase c-Yes. YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |