2x YT medium
SIGMA/Y1003 - Liquid microbial growth medium
Synonym: 2x Yeast Extract Tryptone medium; 2xTY medium; 2xYT Medium
Product Type: Chemical
| application(s) | microbiology |
| form | liquid |
| grade | Molecular Biology |
| pH | 6.5-7.5 |
| Quality Level | 300  |
| sterility | sterile; 0.2 μm filtered |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | 2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added. |
| Features and Benefits: | 2xYT liquid provides: • Filter-sterilized, ready-to-use format • Convenient package size • Standard formulation |
| General description: | 2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. |
| Other Notes: | 16 g/L Tryptone 10 g/L Yeast Extract 5.0 g/L NaCl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106225 |