Yeast Nitrogen Base With Amino Acids
SIGMA/Y1250 - Non-selective yeast growth medium
Synonym: ynb with amino acids
Product Type: Chemical
form | powder |
grade | for molecular biology |
pH | 5.3-5.5(0.67% solution) |
Quality Level | 200 ![]() |
sterility | non-sterile |
storage temp. | room temp |
suitability | nonselective for Candida spp. |
nonselective for Pichia spp. | |
nonselective for Saccharomyces spp. | |
nonselective for Zygosaccharomyces spp. |
Application: | Yeast Nitrogen Base With Amino Acids has been used: • in the ZYP-31 media preparation for E.coli • in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source • to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard • in the preparation of imaging medium Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate. |
Components: | Component, amount (μg/L unless indicated) Nitrogen Sources: Ammonium sulfate, 5.0 g/L Amino Acids: L-histidine, 10 mg/L DL-methionine, 20 mg/L DL-tryptophan, 20 mg/L Vitamins: Biotin, 2.0 Calcium pantothenate, 400 Folic acid, 2.0 Inositol, 2.0 mg/L Nicotinic acid, 400 p-Aminobenzoic acid, 200 Pyridoxine HCl, 400 Riboflavin, 200 Thiamine HCL, 400 Trace Elements: Boric acid Copper sulfate Potassium iodide Ferric chloride Magnesium sulfate Sodium molybdate Zinc sulfate Salts: Potassium phosphate monobasic, 1.0 g/L Magnesium sulfate, 0.5 g/L Sodium chloride, 0.1 g/L Calcium chloride, 0.1 g/L |
General description: | Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts. |
Packaging: | 250 g in poly bottle |
Reconstitution: | Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C. Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | room temp |
UNSPSC | 12352200 |