Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate
SIGMA/Y1251 - suitable for microbiology, NutriSelect® Basic
Product Type: Chemical
| application(s) | clinical testing food and beverages life science and biopharma |
| microbiology | |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| packaging | poly bottle of 1 kg |
| poly bottle of 500 g | |
| Quality Level | 200  |
| solubility | water: soluble 67 mg/mL |
| sterility | non-sterile |
| suitability | Candida spp. |
| Pichia spp. | |
| Saccharomyces spp. | |
| Zygosaccharomyces spp. | |
| molds | |
| yeasts |
| Application: | Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is used in classifying yeasts based on carbon and nitrogen requirements. It contains all essential nutrients required for the growth of yeasts except amino acids, nitrogen and carbohydrate. The addition of nitrogen and carbon sources is required. |
| Components: | Ingredients Biotin, 2 μg/L Calcium pantothenate, 400 μg/L Folic acid, 2 μg/L Niacin, 400 μg/L p-Aminobenzoic acid, 200 μg/L Pyridoxine HCl, 400 μg/L Riboflavin, 200 μg/L Thiamine HCl, 400 μg/L Inositol, 2 mg/L Boric acid, 500 μg/L Copper sulfate, 40 μg/L Potassium iodide, 100 μg/L Ferric chloride, 200 μg/L Manganese sulfate, 400 μg/L Sodium molybdate, 200 μg/L Zinc sulfate, 400 μg/L Potassium phosphate monobasic, 1 g/L Magnesium sulfate, 0.5 g/L Sodium chloride, 0.1 g/L Calcium chloride, 0.1 g/L |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is recommended to use for classifying yeasts based on their carbon and nitrogen requirements. The medium contains all essential nutrients required for the growth of yeasts except amino acids, histidine, methionine and tryptophan, and also ammonium sulphate. The addition of nitrogen and carbon sources is required as described in the Manual of Clinical Microbiology |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100 g in poly bottle |
| Preparation Note: | • Prepare a 10× stock solution by suspending 1.7 g of yeast nitrogen base without amino acids and ammonium sulfate in 100 ml of cold distilled water. Include desired nitrogen and carbon sources. • Warm if necessary to solubilize and sterilize by filtration. • Store 10× stock solution at 2-8°C. To use, dilute 1:10 with sterile distilled water under aspetic conditions. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |
| Components | KH2PO4 1 g/L; MgSO4 .5 g/L; NaCl .1 g/L; CaCl2 .1 g/L; Inositol 2 mg/L |