YPD Broth
SIGMA/Y1375 - suitable for microbiology, NutriSelect® Basic
Synonym: Yeast Peptone Dextrose Broth
Product Type: Chemical
| application(s) | environmental food and beverages |
| microbiology | |
| color | beige |
| composition | Bacteriological peptone, 20 g/L |
| Glucose, 20 g/L | |
| Yeast extract, 10 g/L | |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| packaging | poly bottle of 1 kg |
| poly bottle of 250 g | |
| pH | 6.3-6.7 |
| Quality Level | 200  |
| sterility | non-sterile |
| suitability | nonselective for Candida spp. |
| nonselective for Pichia spp. | |
| nonselective for Saccharomyces spp. | |
| nonselective for Zygosaccharomyces spp. | |
| technique(s) | microbiological culture: suitable |
| Application: | YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 250 g in poly bottle |
| Preparation Note: | 1. Suspend 50 g in 1 L of distilled water. 2. Autoclave for 15 minutes at 121°C. |
| Quantity: | 50 g per liter |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171607 |