Yeast Synthetic Drop-out Medium Supplements
SIGMA/Y1751 - without histidine
MDL Number: MFCD01095679
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: Y1751-20G
| application(s) | food and beverages |
| form | powder |
| Quality Level | 100  |
| Application: | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
| Components: | The supplements contain all of the following components except as indicated: Amino acids: All standard amino acids except for histidine. All the amino acids are present at a concentration of 76 mg/lL except for leucine, which is present at 380 mg/L. Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), uracil (76 mg/L), p-aminobenzoic acid (8 mg/L). |
| Other Notes: | Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids. |
| Packaging: | 20 g in poly bottle |
| Quantity: | 1.92 g will supplement 1 liter of medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12000000 |